IGEM:MIT/2007/Notebook/2007-7-18
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Agenda
- Transform tk's competent cells with yesterday's 3A assembly results (F2620+B0034 and CPX insert into vector pSB1AC3)
- Make LB+Cm and LB+Cm+Kan plates
- Meeting with TK and Drew
Transformed tk's competent cells
- Aliquoted one of the original vials into eight eppendorfs, 100µL each.
- Did 3 sets of transformations:
- 2 of yesterday's 3A assembly product (plasmid contains: F2620+B0034+CPX in pSB1AC3 backbone)
- 1 negative control (pSB1AC3 digested with EcoRI and PstI)
- Followed our Transformation protocol (tk's modified protocol)
Poured Plates, again
- Used stringent working concentrations:
- 10µg of Kanamycin per mL of LB
- 25µg of Chloramphenicol per mL of LB
- Poured 5 LB+Cm+Kan plates
- Poured 29 LB+Cm plates
- Cm plates have green labels