IGEM:MIT/2007/Notebook/2007-7-19: Difference between revisions

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(-) Cm + Kan                      0
(-) Cm + Kan                      0
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==Liquid Culture of BBa_I13500==
* Last night's growth was unsuccessful, so it was redone

Revision as of 07:32, 19 July 2007

Agenda for Thursday, 7/19

  • Make permanent glycerol stocks of 8 tubes
  • AK/AL list
  • Colony PCR on yesterday's plated transformation (F2620+B0034+CPX in pSB1AC3)

Colony Count of Yesterday's Plates

TK's DH5a transformed with F2620+B0034+CPX in pSB1AC3

Key:

  • (+) contains DH5a transformed with digested and ligated parts
  • (-) contains DH5a transformed with only digested and ligated pSB1AC3
  • BH the plate is labeled with Bernice's initials
  • JH the plate is labeled with Jess's initials
Plate                              #Colonies     Notes
(+) Cm        BH                   ~50           A couple satellite colonies
(+) Cm + Kan  BH                   1             This plate hardened imperfectly
(+) Cm        JH                   ~64
(+) Cm + Kan  JH                   3             On the lip of the plate
(-) Cm                             9             
(-) Cm + Kan                       0

Liquid Culture of BBa_I13500

  • Last night's growth was unsuccessful, so it was redone
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