IGEM:MIT/2007/Notebook/2007-7-19

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(Agenda for Thursday, 7/19)
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(-) Cm + Kan                      0
(-) Cm + Kan                      0
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===Permanent Glycerol Stocks of DB3.1, F2620, B0034 and E1010===
 +
*SK: Made eight cryogenic vials of glycerol stock; all have 1 mL 40% glycerol + 1 mL liquid sample
 +
*All vials in the white sticker labeled "iGEM 2007 DH5alpha" -80 degree freezer box
 +
*DB3.1, 2 vials = original with pSB3K3 (K+)
 +
*F2620, 2 vials = transformed (A+)
 +
*B0034, 2 vials = transformed (A+)
 +
*E1010, 2 vials = transformed (K+)
==Liquid Culture of BBa_I13500==
==Liquid Culture of BBa_I13500==
* Last night's growth was unsuccessful, so it was redone
* Last night's growth was unsuccessful, so it was redone

Revision as of 11:45, 19 July 2007

Contents

Agenda for Thursday, 7/19

  • Make permanent glycerol stocks of 8 tubes
  • AK/AL list
  • Colony PCR on yesterday's plated transformation (F2620+B0034+CPX in pSB1AC3)

Colony Count of Yesterday's Plates

TK's DH5a transformed with F2620+B0034+CPX in pSB1AC3

Key:

  • (+) contains DH5a transformed with digested and ligated parts
  • (-) contains DH5a transformed with only digested and ligated pSB1AC3
  • BH the plate is labeled with Bernice's initials
  • JH the plate is labeled with Jess's initials
Plate                              #Colonies     Notes
(+) Cm        BH                   ~50           A couple satellite colonies
(+) Cm + Kan  BH                   1             This plate hardened imperfectly
(+) Cm        JH                   ~64
(+) Cm + Kan  JH                   3             On the lip of the plate
(-) Cm                             9             
(-) Cm + Kan                       0

Permanent Glycerol Stocks of DB3.1, F2620, B0034 and E1010

  • SK: Made eight cryogenic vials of glycerol stock; all have 1 mL 40% glycerol + 1 mL liquid sample
  • All vials in the white sticker labeled "iGEM 2007 DH5alpha" -80 degree freezer box
  • DB3.1, 2 vials = original with pSB3K3 (K+)
  • F2620, 2 vials = transformed (A+)
  • B0034, 2 vials = transformed (A+)
  • E1010, 2 vials = transformed (K+)

Liquid Culture of BBa_I13500

  • Last night's growth was unsuccessful, so it was redone
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