IGEM:MIT/2007/Notebook/2007-7-20: Difference between revisions
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(New page: ==Agenda== *Dilute liquid cultures of Colony PCR 3A(F+B+CPX) #5,6,8 **Check antibiotic resistance screening plates first **Some for Miniprep ***Some of Miniprep for sequencing **Some for g...) |
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*Check about ordering biobrick primers | *Check about ordering biobrick primers | ||
*Streak DH5a for mercury assay | *Streak DH5a for mercury assay | ||
*Transformation of TK Chemically Competent Cells | |||
**1 µl of BioBrick DNA (I13500) | |||
**1 hour incubation in 37 degree room | |||
**Spread 150 µl on A+ plate --> put in warm room at 2.30 PM ("iGEM 2007 7/20/07 TK Cells Transf. I13500") | |||
Revision as of 12:28, 20 July 2007
Agenda
- Dilute liquid cultures of Colony PCR 3A(F+B+CPX) #5,6,8
- Check antibiotic resistance screening plates first
- Some for Miniprep
- Some of Miniprep for sequencing
- Some for glycerol stock
- Some for Polystyrene-binding Assay
- Check about ordering biobrick primers
- Streak DH5a for mercury assay
- Transformation of TK Chemically Competent Cells
- 1 µl of BioBrick DNA (I13500)
- 1 hour incubation in 37 degree room
- Spread 150 µl on A+ plate --> put in warm room at 2.30 PM ("iGEM 2007 7/20/07 TK Cells Transf. I13500")
