IGEM:MIT/2007/Notebook/2007-7-20
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(Difference between revisions)
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*Check about ordering biobrick primers | *Check about ordering biobrick primers | ||
*Streak DH5a for mercury assay | *Streak DH5a for mercury assay | ||
| - | *Transformation of TK Chemically Competent Cells | + | *Transformation of TK Chemically Competent Cells (BH, SK, JH) |
**1 µl of BioBrick DNA (I13500) | **1 µl of BioBrick DNA (I13500) | ||
**1 hour incubation in 37 degree room | **1 hour incubation in 37 degree room | ||
**Spread 150 µl on A+ plate --> put in warm room at 2.30 PM ("iGEM 2007 7/20/07 TK Cells Transf. I13500") | **Spread 150 µl on A+ plate --> put in warm room at 2.30 PM ("iGEM 2007 7/20/07 TK Cells Transf. I13500") | ||
Revision as of 15:28, 20 July 2007
Agenda
- Dilute liquid cultures of Colony PCR 3A(F+B+CPX) #5,6,8
- Check antibiotic resistance screening plates first
- Some for Miniprep
- Some of Miniprep for sequencing
- Some for glycerol stock
- Some for Polystyrene-binding Assay
- Check about ordering biobrick primers
- Streak DH5a for mercury assay
- Transformation of TK Chemically Competent Cells (BH, SK, JH)
- 1 µl of BioBrick DNA (I13500)
- 1 hour incubation in 37 degree room
- Spread 150 µl on A+ plate --> put in warm room at 2.30 PM ("iGEM 2007 7/20/07 TK Cells Transf. I13500")
