IGEM:MIT/2007/Notebook/2007-7-20

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(Agenda)
(Agenda)
Line 8: Line 8:
*Check about ordering biobrick primers
*Check about ordering biobrick primers
*Streak DH5a for mercury assay
*Streak DH5a for mercury assay
-
*Transformation of TK Chemically Competent Cells
+
*Transformation of TK Chemically Competent Cells (BH, SK, JH)
**1 µl of BioBrick DNA (I13500)
**1 µl of BioBrick DNA (I13500)
**1 hour incubation in 37 degree room
**1 hour incubation in 37 degree room
**Spread 150 µl on A+ plate --> put in warm room at 2.30 PM ("iGEM 2007 7/20/07 TK Cells Transf. I13500")
**Spread 150 µl on A+ plate --> put in warm room at 2.30 PM ("iGEM 2007 7/20/07 TK Cells Transf. I13500")

Revision as of 15:28, 20 July 2007

Agenda

  • Dilute liquid cultures of Colony PCR 3A(F+B+CPX) #5,6,8
    • Check antibiotic resistance screening plates first
    • Some for Miniprep
      • Some of Miniprep for sequencing
    • Some for glycerol stock
    • Some for Polystyrene-binding Assay
  • Check about ordering biobrick primers
  • Streak DH5a for mercury assay
  • Transformation of TK Chemically Competent Cells (BH, SK, JH)
    • 1 µl of BioBrick DNA (I13500)
    • 1 hour incubation in 37 degree room
    • Spread 150 µl on A+ plate --> put in warm room at 2.30 PM ("iGEM 2007 7/20/07 TK Cells Transf. I13500")
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