IGEM:MIT/2007/Notebook/2007-7-23: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→Agenda) |
No edit summary |
||
| Line 1: | Line 1: | ||
=Agenda= | |||
#Polystyrene Assay | #Polystyrene Assay | ||
#*Morning: get correct OD | #*Morning: get correct OD | ||
| Line 13: | Line 13: | ||
==Digestion of I13500== | |||
*Made two mixtures | *Made two mixtures | ||
| Line 28: | Line 28: | ||
* Placed in 37C @ 10:20am --> Take out @ 1:20pm | * Placed in 37C @ 10:20am --> Take out @ 1:20pm | ||
==Diluted BioBrick Sequencing Primers== | |||
You can find the stock eppendorfs in the unnamed -20C fridge (Jason+Barry's room). | You can find the stock eppendorfs in the unnamed -20C fridge (Jason+Barry's room). | ||
Revision as of 08:56, 23 July 2007
Agenda
- Polystyrene Assay
- Morning: get correct OD
- Add AHL
- Afternoon: Test
- Digest I13500
- Ligate 3A with I13500 and Mer in pSB1AT3 (so that, if we combine with polystyrene-binding plasmid into a single cell, can test for presence of both plasmids)
- run overnight
- dilute sequencing primers
- sequence PHIE6P
- sequence F2620+B0034+CPX
- Order T7 antibody
Digestion of I13500
- Made two mixtures
4.5µl I13500(Miniprep #1, 223.5ng/µl) 1µl XbaI 1µl PstI 0.5µl BSA 5µl NEB3 Buffer 38µl H20 ------------------------- 50µl Total
- Placed in 37C @ 10:20am --> Take out @ 1:20pm
Diluted BioBrick Sequencing Primers
You can find the stock eppendorfs in the unnamed -20C fridge (Jason+Barry's room).
Diluted from 100µM stock to 32.2µM with total volume of 1.55mL each.
Made 1 VF2 and 1 VR for iGEM use.
