IGEM:MIT/2007/Notebook/2007-7-24

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(New page: ==AGENDA== *Plate the Mer/I13500 3A transformation after overnight ligation (and grow) *Check if sequence results are available from Monday (length was correct) *Second polystyrene assay (...)
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Revision as of 09:39, 24 July 2007

AGENDA

  • Plate the Mer/I13500 3A transformation after overnight ligation (and grow)
  • Check if sequence results are available from Monday (length was correct)
  • Second polystyrene assay (JH and TTP?)
    • CPX, PBS with different concentrations and controls
    • Suggested: one person per plate, pipet to the edge, more washes, harder aspirations
  • Sequence the FhuA (pHIE) with CORRECT primers (which already exist?)
    • Prepped plasmid exists (AL, 7/23)
  • EC_20 requests
    • Harass the people AK e-mailed OR design and order?
  • If T7 antibody comes in...test!
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