IGEM:MIT/2007/Notebook/2007-7-25: Difference between revisions
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==Agenda== | ==Agenda== | ||
#Redo transformation of merR/Pmer+I13500 | #Redo transformation of merR/Pmer+I13500 | ||
#*Plates in @3:25pm | |||
#Make LC of last night's CPX and FhuA transformations into BL21 (eventually will make glycerol stocks) | #Make LC of last night's CPX and FhuA transformations into BL21 (eventually will make glycerol stocks) | ||
#Run western blot on CPX trans. cells (check for protein expression) | #Run western blot on CPX trans. cells (check for protein expression) | ||
#Run assay on FhuA trans. cells (test poly-styrene binding) | #Run assay on FhuA trans. cells (test poly-styrene binding) | ||
===Polystyrene Assay=== | |||
Gr | |||
===merR/Pmer+I13500 Transformation Results=== | ===merR/Pmer+I13500 Transformation Results=== | ||
| Line 20: | Line 24: | ||
F+B+CPX (colony PCR #6) ~200 | F+B+CPX (colony PCR #6) ~200 | ||
</pre> | </pre> | ||
===Second merR/Pmer+I13500 Transformation=== | |||
*Spun down unplated remnants of transformation. Saw a VERY small pellet. | |||
*Resuspended spun-down cells and plated all of those. | |||
===LC of BL21's trans. with F+B+CPX and pICCSP=== | |||
*went in around 4PM | |||
Latest revision as of 12:15, 27 July 2007
Agenda
- Redo transformation of merR/Pmer+I13500
- Plates in @3:25pm
- Make LC of last night's CPX and FhuA transformations into BL21 (eventually will make glycerol stocks)
- Run western blot on CPX trans. cells (check for protein expression)
- Run assay on FhuA trans. cells (test poly-styrene binding)
Polystyrene Assay
Gr
merR/Pmer+I13500 Transformation Results
Plate # colonies Notes - control 0 merR/Pmer+I13500 0 :( + cotrol (pUC18) smear
BL21 Transformation Results
Plate # Colonies Notes FhuA (pICCBP) 38 Colonies are differently sized pUC18 15 2 or 3 are much smaller F+B+CPX (colony PCR #6) ~200
Second merR/Pmer+I13500 Transformation
- Spun down unplated remnants of transformation. Saw a VERY small pellet.
- Resuspended spun-down cells and plated all of those.
LC of BL21's trans. with F+B+CPX and pICCSP
- went in around 4PM
