IGEM:MIT/2007/Notebook/2007-7-25: Difference between revisions
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==Agenda== | ==Agenda== | ||
# | #Redo transformation of merR/Pmer+I13500 | ||
#Make LC of last night's CPX and FhuA transformations into BL21 (eventually will make glycerol stocks) | #Make LC of last night's CPX and FhuA transformations into BL21 (eventually will make glycerol stocks) | ||
#Run western blot on CPX trans. cells (check for protein expression) | #Run western blot on CPX trans. cells (check for protein expression) | ||
Revision as of 07:17, 25 July 2007
Agenda
- Redo transformation of merR/Pmer+I13500
- Make LC of last night's CPX and FhuA transformations into BL21 (eventually will make glycerol stocks)
- Run western blot on CPX trans. cells (check for protein expression)
- Run assay on FhuA trans. cells (test poly-styrene binding)
merR/Pmer+I13500 Transformation Results
Plate # colonies Notes - control 0 merR/Pmer+I13500 0 :( + cotrol (pUC18) smearBL21 Transformation Results
Plate # Colonies Notes FhuA (pICCBP) 38 Colonies are differently sized pUC18 15 2 or 3 are much smaller F+B+CPX (colony PCR #6) ~200
