IGEM:MIT/2007/Notebook/2007-7-26: Difference between revisions
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pUC18 on LB smear | pUC18 on LB smear | ||
pUC18 on Amp+ lawn | pUC18 on Amp+ lawn | ||
</pre> | |||
===Results of Second Plating (yesterday's mer+gfp+1AT3 transformation)=== | |||
*All plates were spread with remainder of transformants | |||
<pre> | |||
Plate # Colonies Notes | |||
1µL DNA 20 smear with some tiny distinct colonies | |||
5µL DNA 21 | |||
10µL DNA 12 | |||
(-) control 0 | |||
pUC18 on LB smear | |||
pUC18 on Amp+ 2880 | |||
</pre> |
Revision as of 09:40, 26 July 2007
Agenda
- Coomassie staining of AHL-induced CPX cells
- Transform the second mer-gfp-1AT3 ligation
- Pick and LC last night's plated transformations (mer+gfp+1AT3)
- Streaked some of Barry's BL21 competent cells
Results of First Plating (yesterday's mer+gfp+1AT3 transformation)
- All plates were spread with 150µL of transformants
Plate # Colonies Notes 1µL DNA 0 5µL DNA 8 10µL DNA 0 (-) control 4 pUC18 on LB smear pUC18 on Amp+ lawn
Results of Second Plating (yesterday's mer+gfp+1AT3 transformation)
- All plates were spread with remainder of transformants
Plate # Colonies Notes 1µL DNA 20 smear with some tiny distinct colonies 5µL DNA 21 10µL DNA 12 (-) control 0 pUC18 on LB smear pUC18 on Amp+ 2880