IGEM:MIT/2007/Notebook/2007-7-26: Difference between revisions
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==Agenda== | ==Agenda== | ||
# | #Western Blot of AHL-induced CPX cells | ||
#Transform the second mer-gfp-1AT3 ligation | #Transform the second mer-gfp-1AT3 ligation | ||
#Pick and LC last night's plated transformations (mer+gfp+1AT3) | #Pick and LC last night's plated transformations (mer+gfp+1AT3) | ||
# | #Streak Barry's BL21 competent cells | ||
#Make glycerol stock of BL21 trans. with F+B+CPX and pICCSP | |||
===Results of First Plating (yesterday's mer+gfp+1AT3 transformation)=== | ===Results of First Plating (yesterday's mer+gfp+1AT3 transformation)=== |
Latest revision as of 12:52, 26 July 2007
Agenda
- Western Blot of AHL-induced CPX cells
- Transform the second mer-gfp-1AT3 ligation
- Pick and LC last night's plated transformations (mer+gfp+1AT3)
- Streak Barry's BL21 competent cells
- Make glycerol stock of BL21 trans. with F+B+CPX and pICCSP
Results of First Plating (yesterday's mer+gfp+1AT3 transformation)
- All plates were spread with 150µL of transformants
Plate # Colonies Notes 1µL DNA 0 5µL DNA 8 10µL DNA 0 (-) control 4 pUC18 on LB smear pUC18 on Amp+ lawn
Results of Second Plating (yesterday's mer+gfp+1AT3 transformation)
- All plates were spread with remainder of transformants
Plate # Colonies Notes 1µL DNA 20 smear with some tiny distinct colonies 5µL DNA 21 10µL DNA 12 (-) control 0 pUC18 on LB smear pUC18 on Amp+ 2880