IGEM:MIT/2007/Notebook/2007-7-26

Agenda

1. Coomassie staining of AHL-induced CPX cells
2. Transform the second mer-gfp-1AT3 ligation
3. Pick and LC last night's plated transformations (mer+gfp+1AT3)
4. Streaked some of Barry's BL21 competent cells

Results of First Plating (yesterday's mer+gfp+1AT3 transformation)

• All plates were spread with 150µL of transformants

Plate          # Colonies      Notes
1µL DNA        0
5µL DNA        8
10µL DNA       0
(-) control    4
pUC18 on LB    smear
pUC18 on Amp+  lawn

Results of Second Plating (yesterday's mer+gfp+1AT3 transformation)

• All plates were spread with remainder of transformants

Plate          # Colonies      Notes
1µL DNA        20              smear with some tiny distinct colonies
5µL DNA        21
10µL DNA       12
(-) control    0
pUC18 on LB    smear
pUC18 on Amp+  2880