IGEM:MIT/2007/Notebook/2007-7-26

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Agenda

  1. Western Blot of AHL-induced CPX cells
  2. Transform the second mer-gfp-1AT3 ligation
  3. Pick and LC last night's plated transformations (mer+gfp+1AT3)
  4. Streak Barry's BL21 competent cells
  5. Make glycerol stock of BL21 trans. with F+B+CPX and pICCSP

Results of First Plating (yesterday's mer+gfp+1AT3 transformation)

  • All plates were spread with 150µL of transformants
Plate          # Colonies      Notes
1µL DNA        0
5µL DNA        8
10µL DNA       0
(-) control    4
pUC18 on LB    smear
pUC18 on Amp+  lawn

Results of Second Plating (yesterday's mer+gfp+1AT3 transformation)

  • All plates were spread with remainder of transformants
Plate          # Colonies      Notes
1µL DNA        20              smear with some tiny distinct colonies
5µL DNA        21
10µL DNA       12
(-) control    0
pUC18 on LB    smear
pUC18 on Amp+  2880
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