IGEM:MIT/2007/Notebook/2007-7-27: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
Line 14: | Line 14: | ||
(Done in Triplicate) | (Done in Triplicate) | ||
CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced) | CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced) | ||
</pre> | |||
==Western Blot Results== | ==Western Blot Results== | ||
[[Media:070727_iGEM2007_t7_fusions.pdf|A pdf of the blots]] | [[Media:070727_iGEM2007_t7_fusions.pdf|A pdf of the blots]] |
Revision as of 08:55, 31 July 2007
Agenda
- dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
- Miniprep LC's of Mer/GFP
- Finish Western Blot
- Order EC20 oligos
EC_20 Oligos
- Ordered with IE-HCLG purification, should arrive Tuesday
Polystyrene Assay
Wells: (Done in Triplicate) CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced)