IGEM:MIT/2007/Notebook/2007-7-27: Difference between revisions

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(New page: ==Agenda== *dilute LC's of Mer/GFP Transformations and BL21 untransformed cells **Miniprep LC's of Mer/GFP *Finish Western Blot *Order EC20 oligos)
 
 
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==Agenda==
=Agenda=
*dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
*dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
**Miniprep LC's of Mer/GFP
**Miniprep LC's of Mer/GFP
*Finish Western Blot
*Finish Western Blot
*Order EC20 oligos
*Order EC20 oligos
==EC_20 Oligos==
* Ordered with IE-HCLG purification, should arrive Tuesday
==Polystyrene Assay==
<pre>
Wells:
(Done in Triplicate)
CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced)
</pre>
[[Image:Polystyreneassay.jpg|thumb|Diagram of the Polystyrene Assay Protocol]]
<gallery>
Image:E-5a.jpg|E-5a
Image:E-5b.jpg|E-5b
Image:E-5c.jpg|E-5c
Image:E-6a.jpg|E-6a
Image:E-6b.jpg|E-6b
Image:E-6c.jpg|E-6c
Image:E-7a.jpg|E-7a
Image:E-7b.jpg|E-7b
Image:E-7c.jpg|E-7c
Image:E-7d.jpg|E-7d
Image:E-7e.jpg|E-7e
Image:E-7f.jpg|E-7f
Image:E-8a.jpg|E-8a
Image:E-8b.jpg|E-8b
Image:E-8c.jpg|E-8c
Image:E-8d.jpg|E-8d
Image:E-8e.jpg|E-8e
Image:E-8f.jpg|E-8f
Image:Control1.jpg|Control 1
Image:Control2.jpg|Control 2
Image:Control3.jpg|Control 3
Image:Image21.jpg|Image 21
</gallery>
[[Image:Polystyreneassay.jpg|thumb|Diagram of the Polystyrene Assay Protocol]]
==Western Blot Results==
[[Media:070727_iGEM2007_t7_fusions.pdf|A pdf of the blots]]

Latest revision as of 10:17, 17 August 2007

Agenda

  • dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
    • Miniprep LC's of Mer/GFP
  • Finish Western Blot
  • Order EC20 oligos

EC_20 Oligos

  • Ordered with IE-HCLG purification, should arrive Tuesday

Polystyrene Assay

Wells:
(Done in Triplicate)
CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced)
Diagram of the Polystyrene Assay Protocol
  • E-5a

    E-5a

  • E-5b

    E-5b

  • E-5c

    E-5c

  • E-6a

    E-6a

  • E-6b

    E-6b

  • E-6c

    E-6c

  • E-7a

    E-7a

  • E-7b

    E-7b

  • E-7c

    E-7c

  • E-7d

    E-7d

  • E-7e

    E-7e

  • E-7f

    E-7f

  • E-8a

    E-8a

  • E-8b

    E-8b

  • E-8c

    E-8c

  • E-8d

    E-8d

  • E-8e

    E-8e

  • E-8f

    E-8f

  • Control 1

    Control 1

  • Control 2

    Control 2

  • Control 3

    Control 3

  • Image 21

    Image 21

Diagram of the Polystyrene Assay Protocol

Western Blot Results

A pdf of the blots

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