IGEM:MIT/2007/Notebook/2007-7-27

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New page: ==Agenda== *dilute LC's of Mer/GFP Transformations and BL21 untransformed cells **Miniprep LC's of Mer/GFP *Finish Western Blot *Order EC20 oligos)
(Agenda)
Line 1: Line 1:
-
==Agenda==
+
=Agenda=
*dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
*dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
**Miniprep LC's of Mer/GFP
**Miniprep LC's of Mer/GFP
*Finish Western Blot
*Finish Western Blot
*Order EC20 oligos
*Order EC20 oligos
 +
 +
==EC_20 Oligos==
 +
* Ordered with IE-HCLG purification, should arrive Tuesday
 +
 +
==Polystyrene Assa=
 +
 +
<pre>
 +
Wells:
 +
(Done in Triplicate)
 +
CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced)

Revision as of 15:36, 27 July 2007

Agenda

  • dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
    • Miniprep LC's of Mer/GFP
  • Finish Western Blot
  • Order EC20 oligos

EC_20 Oligos

  • Ordered with IE-HCLG purification, should arrive Tuesday

=Polystyrene Assa

Wells:
(Done in Triplicate)
CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced)
Personal tools