IGEM:MIT/2007/Notebook/2007-7-27

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(Polystyrene Assay)
(Polystyrene Assay)
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Image:Image21.jpg|Image 21
Image:Image21.jpg|Image 21
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[[Image:Polystyreneassay.jpg|thumb|Diagram of the Polystyrene Assay Protocol]]
==Western Blot Results==
==Western Blot Results==
[[Media:070727_iGEM2007_t7_fusions.pdf|A pdf of the blots]]
[[Media:070727_iGEM2007_t7_fusions.pdf|A pdf of the blots]]

Revision as of 13:17, 17 August 2007

Contents

Agenda

  • dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
    • Miniprep LC's of Mer/GFP
  • Finish Western Blot
  • Order EC20 oligos

EC_20 Oligos

  • Ordered with IE-HCLG purification, should arrive Tuesday

Polystyrene Assay

Wells:
(Done in Triplicate)
CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced)


Diagram of the Polystyrene Assay Protocol
Diagram of the Polystyrene Assay Protocol

Western Blot Results

A pdf of the blots

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