IGEM:MIT/2007/Notebook/2007-7-27

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Agenda

  • dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
    • Miniprep LC's of Mer/GFP
  • Finish Western Blot
  • Order EC20 oligos

EC_20 Oligos

  • Ordered with IE-HCLG purification, should arrive Tuesday

Polystyrene Assay

Wells:
(Done in Triplicate)
CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced)
Diagram of the Polystyrene Assay Protocol
  • E-5a

    E-5a

  • E-5b

    E-5b

  • E-5c

    E-5c

  • E-6a

    E-6a

  • E-6b

    E-6b

  • E-6c

    E-6c

  • E-7a

    E-7a

  • E-7b

    E-7b

  • E-7c

    E-7c

  • E-7d

    E-7d

  • E-7e

    E-7e

  • E-7f

    E-7f

  • E-8a

    E-8a

  • E-8b

    E-8b

  • E-8c

    E-8c

  • E-8d

    E-8d

  • E-8e

    E-8e

  • E-8f

    E-8f

  • Control 1

    Control 1

  • Control 2

    Control 2

  • Control 3

    Control 3

  • Image 21

    Image 21

Diagram of the Polystyrene Assay Protocol

Western Blot Results

A pdf of the blots

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