IGEM:MIT/2007/Notebook/2007-7-3: Difference between revisions
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#*Wait until cool to touch and put in 960 µL Kanamycin to make 10 µg/ml concentration | #*Wait until cool to touch and put in 960 µL Kanamycin to make 10 µg/ml concentration | ||
#*Pour into labeled plates | #*Pour into labeled plates | ||
==Incubate 5 colonies of DB3.1 strain with pSB3k3 plasmid (with Kan+)== | |||
*Started at 6pm (spinning in hot room) | |||
** +18 hours = done 12pm tomorrow | |||
==Incubated plates of DH5a== | ==Incubated plates of DH5a== | ||
*Francoise kindly gave us two plates of untransformed DH5a because our own line is probably comprimised | *Francoise kindly gave us two plates of untransformed DH5a because our own line is probably comprimised | ||
*Incubated plates at 37C overnight | *Incubated plates at 37C overnight | ||
Revision as of 06:46, 5 July 2007
Agenda for July 3
- Call Geneart
- Results of Alex/Diti PCR?
- For metals: RBS and promoter? (clarify!)
- Barry's promoter -- obtainment?
- Discuss results of Monday's 2PM meeting
- Set up meeting with Drew/Tom/grads?
Wet Work
- All plates look like sludge
- Not sure why
- Could be need to use LB+KAN for recovery step
- Plating with 1000 µl instead of 100 µl
Make LB+KAN agar plates
- Heat LB+KAN agar plates
- Heat LB + Agar 1.2% for 10 minutes at 50% power
- Wait until cool to touch and put in 960 µL Kanamycin to make 10 µg/ml concentration
- Pour into labeled plates
Incubate 5 colonies of DB3.1 strain with pSB3k3 plasmid (with Kan+)
- Started at 6pm (spinning in hot room)
- +18 hours = done 12pm tomorrow
Incubated plates of DH5a
- Francoise kindly gave us two plates of untransformed DH5a because our own line is probably comprimised
- Incubated plates at 37C overnight
