IGEM:MIT/2007/Notebook/2007-7-3

Agenda for July 3

• Call Geneart
• Results of Alex/Diti PCR?
• For metals: RBS and promoter? (clarify!)
• Barry's promoter -- obtainment?
• Discuss results of Monday's 2PM meeting
• Set up meeting with Drew/Tom/grads?

Wet Work

• All plates look like sludge
  • Not sure why
  • Could be need to use LB+KAN for recovery step
  • Plating with 1000 µl instead of 100 µl

Make LB+KAN agar plates

1. Heat LB+KAN agar plates
   • Heat LB + Agar 1.2% for 10 minutes at 50% power
   • Wait until cool to touch and put in 960 µL Kanamycin to make 10 µg/ml concentration
   • Pour into labeled plates

Incubate 5 colonies of DB3.1 strain with pSB3k3 plasmid (with Kan+)

• Started at 6pm (spinning in hot room)
  • +18 hours = done 12pm tomorrow

Incubated plates of DH5a

• Francoise kindly gave us two plates of untransformed DH5a because our own line is probably comprimised
• Incubated plates at 37C overnight