IGEM:MIT/2007/Notebook/2007-7-30

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(New page: ==Mer-GFP Expression Protocol== # Mix Diluted LB Media (LB4.10) ##4g NaCl, .5g Yeast Exract, 1g Tryptone + 1L H20 # Autoclave LB4.10 # Grow up 10 mls ON at 30°C # Dilute back 1:100 in fr...)
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Revision as of 12:30, 30 July 2007

Mer-GFP Expression Protocol

  1. Mix Diluted LB Media (LB4.10)
    1. 4g NaCl, .5g Yeast Exract, 1g Tryptone + 1L H20
  2. Autoclave LB4.10
  3. Grow up 10 mls ON at 30°C
  4. Dilute back 1:100 in fresh LB4.10
  5. Add HgCl2 in following concentrations (ng/ml):
    1. 0
    2. 5
    3. 10
    4. 25
    5. 50
    6. 75
    7. 100
    8. 150
    9. 300
    10. 500
    11. 1000
    12. 10000
  6. Grow at 30°C for 16h
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