IGEM:MIT/2007/Notebook/2007-7-31: Difference between revisions
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==CPX Immunostaining== | ==CPX Immunostaining== | ||
*Induced cells at 0.1 OD | |||
**10 samples total: 2 each for 0(- control), 10^-8, 10^-7, 10^-6, 10^-5 concentration AHL | |||
*Changed secondary Goat Anti-Mouse antibody concentration to (1:200) | *Changed secondary Goat Anti-Mouse antibody concentration to (1:200) | ||
Revision as of 15:42, 31 July 2007
Agenda
- Run mercury assay
- Redo live immunostaining with altered protocol
- Enter working parts into BioBricks Registry
- Find constitutive promoter and perform 3A assembly with a RBS and CPX
- Redo transformation of BL21 w/ mer.I13500 in 1AT3
Mercury Assay
CPX Assay
Varied cell concentrations: (OD)
- .1
- .3
- .5
- 1
CPX Immunostaining
- Induced cells at 0.1 OD
- 10 samples total: 2 each for 0(- control), 10^-8, 10^-7, 10^-6, 10^-5 concentration AHL
- Changed secondary Goat Anti-Mouse antibody concentration to (1:200)
Transformation of mer.I13500 in BL21
- Nothing grew on any of the plates (not even positive control with pUC18)
- Perhaps cells not competent?
- We should redo tomorrow
