IGEM:MIT/2007/Notebook/2007-7-4: Difference between revisions

Latest revision as of 06:45, 5 July 2007

mini-prep pSB3k3 from DB3.1 from incubation yesterday
streak some DB3.1 for future use
Pick colonies, incubate liquid cultures of DH(alpha)5 - to be taken out of hot room at 4am tonight

1. 8.4 ng/µL
2. 13.1 ng/µL
3. 6.5 ng/µL
4. 11.2 ng/µL
5. 8.1 ng/µL

Two 10 mL liquid cultures of singly colony DH5a (picked from last night's incubated plates) were put into the 37C room to grow overnight

@@ Line 3: / Line 3: @@
 *streak some DB3.1 for future use
 * Pick colonies, incubate liquid cultures of DH(alpha)5 - to be taken out of hot room at 4am tonight
+==Took out DB3.1 liquid cultures==
+*Yay, all five tubes have growth
+*Took from all five cultures for miniprep
+*Will streak from cultures 1, 2, 3
 ==Mini-Prep - Nanodrop of purified pSB3k3==
+<pre>
 . 8.4 ng/µL
 . 13.1 ng/µL
@@ Line 10: / Line 16: @@
 . 11.2 ng/µL
 . 8.1 ng/µL
+</pre>
+==Streaked DB3.1==
+*DB3.1 is the strain that is resistant to the ccdB toxin (
+*Streaked three plates
+*Left in 37C room to incubate overnight
+==Incubated liquid cultures of DH5a==
+*Two 10 mL liquid cultures of singly colony DH5a (picked from last night's incubated plates) were put into the 37C room to grow overnight