IGEM:MIT/2007/Notebook/2007-7-5: Difference between revisions
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(New page: ==Agenda== #Prepare chemically competent cells from the DH5a line #Make long term glycerol stock of DH5a ==DH5a Incubation== *both 10 mL overnight cultures had growth ==Making long term ...) |
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*TSS is located in the -4C fridge (Lester) | *TSS is located in the -4C fridge (Lester) | ||
*Followed OWW protocol http://openwetware.org/wiki/Preparing_chemically_competent_cells | *Followed OWW protocol http://openwetware.org/wiki/Preparing_chemically_competent_cells | ||
Revision as of 06:51, 5 July 2007
Agenda
- Prepare chemically competent cells from the DH5a line
- Make long term glycerol stock of DH5a
DH5a Incubation
- both 10 mL overnight cultures had growth
Making long term glycerol stock of DH5a
Preparing Chemically Competent Cells
- TSS is located in the -4C fridge (Lester)
- Followed OWW protocol http://openwetware.org/wiki/Preparing_chemically_competent_cells