IGEM:MIT/2007/Notebook/2007-7-5: Difference between revisions
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==Making long term glycerol stock of DH5a== | ==Making long term glycerol stock of DH5a== | ||
*Followed OWW protocol: http://www.openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria | |||
*Method: | |||
** Add 1 ml of 40% glycerol in H2O to a cryogenic vial. | |||
** Add 1 ml sample from the culture of bacteria to be stored. | |||
** Gently vortex the cryogenic vial to ensure the culture and glycerol is well-mixed. | |||
*** Alternatively, pipet to mix. | |||
** Use a tough spot to put the name of the strain or some useful identifier on the top of the vial. | |||
** On the side of the vial list all relevant information - part, vector, strain, date, researcher, etc. | |||
** Store in a freezer box in a -80C freezer. Remember to record where the vial is stored for fast retrieval later. | |||
==Preparing Chemically Competent Cells== | ==Preparing Chemically Competent Cells== | ||
*TSS is located in the -4C fridge (Lester) | *TSS is located in the -4C fridge (Lester) | ||
*Followed OWW protocol http://openwetware.org/wiki/Preparing_chemically_competent_cells | *Followed OWW protocol http://openwetware.org/wiki/Preparing_chemically_competent_cells | ||
Revision as of 06:52, 5 July 2007
Agenda
- Prepare chemically competent cells from the DH5a line
- Make long term glycerol stock of DH5a
DH5a Incubation
- both 10 mL overnight cultures had growth
Making long term glycerol stock of DH5a
- Followed OWW protocol: http://www.openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria
- Method:
- Add 1 ml of 40% glycerol in H2O to a cryogenic vial.
- Add 1 ml sample from the culture of bacteria to be stored.
- Gently vortex the cryogenic vial to ensure the culture and glycerol is well-mixed.
- Alternatively, pipet to mix.
- Use a tough spot to put the name of the strain or some useful identifier on the top of the vial.
- On the side of the vial list all relevant information - part, vector, strain, date, researcher, etc.
- Store in a freezer box in a -80C freezer. Remember to record where the vial is stored for fast retrieval later.
Preparing Chemically Competent Cells
- TSS is located in the -4C fridge (Lester)
- Followed OWW protocol http://openwetware.org/wiki/Preparing_chemically_competent_cells
