IGEM:MIT/2007/Notebook/2007-7-7: Difference between revisions

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(New page: ==Agenda== #Pick plates and make liquid cultures ==Checked plates== <pre> B0034 (RBS) plated on 7/5/07 Plate # Colonies Notes 1 19 Overgrown, satellite colonie...)
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Revision as of 06:27, 7 July 2007

Agenda

  1. Pick plates and make liquid cultures

Checked plates

B0034 (RBS) plated on 7/5/07

Plate    # Colonies     Notes
1        19             Overgrown, satellite colonies
2        27             Overgrown, satellite colonies are starting to get too big
3        10             Satellite colonies, didn't count ones I wasn't sure of
E1010 (eRFP) plated on 7/5/07

Plate    # Colonies     Notes
1        8              Counted the ones big enough to be picked.  A lot of little speckles?
2        5              Counted the ones big enough to be picked
3        3              Counted the ones big enough to be picked
F2620 (promoter) plated on 7/5/07

Plate    # Colonies     Notes
1        13             Counted distinct ones
2        5
3        25             Satellite colonies
Positive Controls

Plate                 # Colonies        Notes
PUC18 in LB+Amp       21                Colonies are pretty small, same as yesterday morning's.  Really small satellite colonies
PUC18 in LB           Totally covered