IGEM:MIT/2007/Notebook/2007-7-7: Difference between revisions
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(New page: ==Agenda== #Pick plates and make liquid cultures ==Checked plates== <pre> B0034 (RBS) plated on 7/5/07 Plate # Colonies Notes 1 19 Overgrown, satellite colonie...) |
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==Checked plates== | ==Checked plates== | ||
At 9 AM | |||
<pre> | <pre> | ||
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<pre> | <pre> | ||
Positive Controls | Positive Controls plated on 7/6/07 | ||
Plate # Colonies Notes | Plate # Colonies Notes | ||
PUC18 in LB+Amp 21 Colonies are pretty small, same as yesterday morning's. Really small satellite colonies | PUC18 in LB+Amp 21 Colonies are pretty small, same as yesterday morning's. Really small satellite colonies | ||
PUC18 in LB Totally covered | PUC18 in LB Totally covered | ||
</pre> | |||
<pre> | |||
B0034 (RBS) plated 7/6/07 | |||
Specks, need to incubate longer before inoculating | |||
Plate # Colonies Notes | |||
1 ~100 Smear in the middle | |||
2 ~90 These were the ones I could see, I think there are a bunch growing on the sides | |||
3 ~140 Two smears | |||
</pre> | |||
<pre> | |||
F2620 (promoter) plated 7/6/07 | |||
Specks, need to incubate longer before inoculating | |||
Plate # Colonies Notes | |||
1 32 | |||
2 29 | |||
3 65 | |||
</pre> | |||
<pre> | |||
E1010 (eRFP) plated 7/6/07 | |||
Plate # Colonies Notes | |||
1 27 There's an almost smear. Didn't count tiny specks | |||
2 23 Didn't count tiny specks | |||
3 37 " " | |||
</pre> | |||
==Inoculating== | |||
Picked two colonies from and inoculated in liquid culture the following: | |||
*7/5/07 plated B0034, F2620, and E1010 | |||
*7/6/07 plated E1010 | |||
Left in 37C room to incubate overnight | |||
Tubes have white tape tags that read: | |||
<pre> | |||
iGEM 7/7/07 LB+[antibiotic] | |||
[Part #] in strain DH5a | |||
from [source plate date] [source plate #] [pick#] | |||
</pre> | </pre> | ||
Revision as of 09:00, 7 July 2007
Agenda
- Pick plates and make liquid cultures
Checked plates
At 9 AM
B0034 (RBS) plated on 7/5/07 Plate # Colonies Notes 1 19 Overgrown, satellite colonies 2 27 Overgrown, satellite colonies are starting to get too big 3 10 Satellite colonies, didn't count ones I wasn't sure of
E1010 (eRFP) plated on 7/5/07 Plate # Colonies Notes 1 8 Counted the ones big enough to be picked. A lot of little speckles? 2 5 Counted the ones big enough to be picked 3 3 Counted the ones big enough to be picked
F2620 (promoter) plated on 7/5/07 Plate # Colonies Notes 1 13 Counted distinct ones 2 5 3 25 Satellite colonies
Positive Controls plated on 7/6/07 Plate # Colonies Notes PUC18 in LB+Amp 21 Colonies are pretty small, same as yesterday morning's. Really small satellite colonies PUC18 in LB Totally covered
B0034 (RBS) plated 7/6/07 Specks, need to incubate longer before inoculating Plate # Colonies Notes 1 ~100 Smear in the middle 2 ~90 These were the ones I could see, I think there are a bunch growing on the sides 3 ~140 Two smears
F2620 (promoter) plated 7/6/07 Specks, need to incubate longer before inoculating Plate # Colonies Notes 1 32 2 29 3 65
E1010 (eRFP) plated 7/6/07 Plate # Colonies Notes 1 27 There's an almost smear. Didn't count tiny specks 2 23 Didn't count tiny specks 3 37 " "
Inoculating
Picked two colonies from and inoculated in liquid culture the following:
- 7/5/07 plated B0034, F2620, and E1010
- 7/6/07 plated E1010
Left in 37C room to incubate overnight Tubes have white tape tags that read:
iGEM 7/7/07 LB+[antibiotic] [Part #] in strain DH5a from [source plate date] [source plate #] [pick#]
