- Pick plates and make liquid cultures
Checked plates 9 AM
Smears eeeeverywhere. A lot of growth on last night's plates.
B0034 (RBS) plated on 7/5/07 Plate # Colonies Notes 1 19 Overgrown, satellite colonies 2 27 Overgrown, satellite colonies are starting to get too big 3 10 Satellite colonies, didn't count ones I wasn't sure of
E1010 (eRFP) plated on 7/5/07 Plate # Colonies Notes 1 8 Counted the ones big enough to be picked. A lot of little speckles? 2 5 Counted the ones big enough to be picked 3 3 Counted the ones big enough to be picked
F2620 (promoter) plated on 7/5/07 Plate # Colonies Notes 1 13 Counted distinct ones 2 5 3 25 Satellite colonies
Positive Controls plated on 7/6/07 Plate # Colonies Notes PUC18 in LB+Amp 21 Colonies are pretty small, same as yesterday morning's. Really small satellite colonies PUC18 in LB Totally covered
B0034 (RBS) plated 7/6/07 Specks, need to incubate longer before inoculating Plate # Colonies Notes 1 ~100 Smear in the middle 2 ~90 These were the ones I could see, I think there are a bunch growing on the sides 3 ~140 Two smears
F2620 (promoter) plated 7/6/07 Specks, need to incubate longer before inoculating Plate # Colonies Notes 1 32 2 29 3 65
E1010 (eRFP) plated 7/6/07 Plate # Colonies Notes 1 27 There's an almost smear. Didn't count tiny specks 2 23 Didn't count tiny specks 3 37 " "
Picked two colonies from and inoculated in liquid culture the following:
- 7/5/07 plated B0034, F2620, and E1010
- 7/6/07 plated E1010
11:30 AM, put cultures in 37C room to incubate overnight
Tubes have white tape tags that read:
iGEM 7/7/07 LB+[antibiotic] [Part #] in strain DH5a from plate [source plate date] [source plate #] [pick#]
iGEM 7/7/07 LB+Kan E1010 in strain DH5a from plate 7/5/07 #2 pick 2 This label means: The test tube contains LB + Kanamycin The test tube contains bacteria with biobrick part BBa_E1010 The source plate is labeled with the date 7/5/07 #2 and the corresponding biobrick part The test tube contains the second colony picked from the source plate
Sorry if this is confusing guys, our source plates don't have a naming system yet.
Checked Plates 5 PM
Colonies look bigger
Smears are probably from leaving a puddle after plating.
F2620 from 7/6/07 Plate # Colonies Notes 1 53 2 31 3 ~60 Smear not counted
B0034 from 7/6/07 Plate # Colonies Notes 1 ~40 Smears not counted 2 120 Satellites not counted 3 ~60 Smears not counted
Making liquid cultures 5 PM
- Made liquid cultures in big test tubes of two colonies from each plate (7.6 #1, #2 and #3 of F2620 and B0034 -- both Amp) -- 12 tubes total
- Put in 37° room on rotater for overnight incubation
- Random Note: iGEM team is authorized to pick up the lab phone! (Just in case...Jess and I didn't pick up because we didn't know if we were allowed to today, and Francois was actually locked out of lab for a bit...oops. Sorry.)