IGEM:MIT/2007/Notebook/2007-7-8
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Miniprep of F2620, B0034, E1010 From 7/5/07 Plating
Part(Plate #/ Pick#/ Nanodrop)
B0034 #1 Plate 2/ Part 2/ 84.4 ng/µl B0034 #2 * 3/ 1/ 84.9 ng/µl F2620 #1 * 3/ 2/ 134.5 ng/µl F2620 #2 * 2/ 2/ 113.6 ng/µl E1010 #1 * 1/ 2/ 162.8 ng/µl E1010 #2 * 2/ 1/ 121.5 ng/µl
Digestion of F2620, B0034, pSB3k3
===F2620=== 8.8 µl F2620 0.5 µl BSA 5 µl NEB2 Buffer 2 µl EcoRI 2 µl SpeI 31.7 µl H20 ===B0034=== 11.8 µl B0034 0.5 µl BSA 5 µl NEB3 Buffer 2 µl XbaI 2 µl PstI 28.7 µl H20 ===pSB3k3=== 38.2 µl pSB3k3 0.5 µl BSA 5 µl NEB2 Buffer 2 µl EcoRI 2 µl SpeI 2.3 µl H20
- Add Enzymes Last