IGEM:MIT/2007/Notebook/2007-8-1: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 15: Line 15:
# Put 150ul in plate reader
# Put 150ul in plate reader


 
====Results====
<gallery>
<gallery>
Image:8-1-AHL-5Ai.jpg|AHL 5Ai
Image:8-1-AHL-5Ai.jpg|AHL 5Ai

Revision as of 08:08, 2 August 2007

Agenda

  1. Decide on constitutive promoter for CPX
    • Transform promoter into DH5a
  2. Check sequencing results for Mer/GFP 3A
  3. Perform flourescence assay on mer-gfp construct


Flourescence Assay Protocol

  1. Draw sample of 1.5 ml from incubated cells with Hg
  2. Plot OD's
  3. Spin down 3 mins at 13000 rpm
  4. Aspirate and resuspend in 1.5 ml .9% NaCl
  5. Repeat 3
  6. Repeat 4
  7. Put 150ul in plate reader

Results

  • AHL 5Ai

    AHL 5Ai

  • AHL 5Aii

    AHL 5Aii

  • AHL 5Bi

    AHL 5Bi

  • AHL 5Bii

    AHL 5Bii

  • AHL 6Ai

    AHL 6Ai

  • AHL 6Aii

    AHL 6Aii

  • AHL 6Bi

    AHL 6Bi

  • AHL 7Ai

    AHL 7Ai

  • AHL 7Aii

    AHL 7Aii

  • AHL 7Aiii

    AHL 7Aiii

  • AHL 7Bi

    AHL 7Bi

  • AHL 7Bii

    AHL 7Bii

  • AHL 8Ai

    AHL 8Ai

  • AHL 8Aii

    AHL 8Aii

  • AHL 8Bi

    AHL 8Bi

  • AHL 8Bii

    AHL 8Bii

  • Control Ai

    Control Ai

  • Control Aii

    Control Aii

  • Control Bi

    Control Bi

  • Control Bii

    Control Bii

Sequencing Results

  • mer.I13500 in pSB1AT3 came back really crappy. Biobrick prefix and suffix are partially present, cannot locate the sequence for GFP.
  • Need to send in the parts for sequencing.
  • Will run a colony PCR as a diagnostic.
Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:MIT/2007/Notebook/2007-8-1&oldid=137067"