IGEM:MIT/2007/Notebook/2007-8-1: Difference between revisions
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# Put 150ul in plate reader | # Put 150ul in plate reader | ||
===Sequencing Results=== | ===Sequencing Results=== |
Revision as of 14:10, 2 August 2007
Agenda
- Decide on constitutive promoter for CPX
- Transform promoter into DH5a
- Check sequencing results for Mer/GFP 3A
- Perform flourescence assay on mer-gfp construct
Flourescence Assay Protocol
- Draw sample of 1.5 ml from incubated cells with Hg
- Plot OD's
- Spin down 3 mins at 13000 rpm
- Aspirate and resuspend in 1.5 ml .9% NaCl
- Repeat 3
- Repeat 4
- Put 150ul in plate reader
Sequencing Results
- mer.I13500 in pSB1AT3 came back really crappy. Biobrick prefix and suffix are partially present, cannot locate the sequence for GFP.
- Need to send in the parts for sequencing.
- Will run a colony PCR as a diagnostic.