IGEM:MIT/2007/Notebook/2007-8-1

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(New page: ==Agenda== *Decide on constitutive promoter for CPX **Transform promoter into DH5a *Check sequencing results for Mer/GFP 3A)
(Agenda)
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==Agenda==
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=Agenda=
*Decide on constitutive promoter for CPX
*Decide on constitutive promoter for CPX
**Transform promoter into DH5a
**Transform promoter into DH5a
*Check sequencing results for Mer/GFP 3A
*Check sequencing results for Mer/GFP 3A
 +
*Perform flourescence assay on mer-gfp construct
 +
 +
==Flourescence Assay Protocol==
 +
# Draw sample of 1.5 ml from incubated cells with Hg
 +
# Plot OD's
 +
# Spin down 3 mins at 13000 rpm
 +
# Aspirate and resuspend in 1.5 ml .9% NaCl
 +
# Repeat 3
 +
# Repeat 4
 +
# Put 150ul in plate reader

Revision as of 10:16, 1 August 2007

Agenda

  • Decide on constitutive promoter for CPX
    • Transform promoter into DH5a
  • Check sequencing results for Mer/GFP 3A
  • Perform flourescence assay on mer-gfp construct

Flourescence Assay Protocol

  1. Draw sample of 1.5 ml from incubated cells with Hg
  2. Plot OD's
  3. Spin down 3 mins at 13000 rpm
  4. Aspirate and resuspend in 1.5 ml .9% NaCl
  5. Repeat 3
  6. Repeat 4
  7. Put 150ul in plate reader
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