IGEM:MIT/2007/Notebook/2007-8-1: Difference between revisions
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(New page: ==Agenda== *Decide on constitutive promoter for CPX **Transform promoter into DH5a *Check sequencing results for Mer/GFP 3A) |
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=Agenda= | |||
*Decide on constitutive promoter for CPX | *Decide on constitutive promoter for CPX | ||
**Transform promoter into DH5a | **Transform promoter into DH5a | ||
*Check sequencing results for Mer/GFP 3A | *Check sequencing results for Mer/GFP 3A | ||
*Perform flourescence assay on mer-gfp construct | |||
==Flourescence Assay Protocol== | |||
# Draw sample of 1.5 ml from incubated cells with Hg | |||
# Plot OD's | |||
# Spin down 3 mins at 13000 rpm | |||
# Aspirate and resuspend in 1.5 ml .9% NaCl | |||
# Repeat 3 | |||
# Repeat 4 | |||
# Put 150ul in plate reader | |||
Revision as of 07:16, 1 August 2007
Agenda
- Decide on constitutive promoter for CPX
- Transform promoter into DH5a
- Check sequencing results for Mer/GFP 3A
- Perform flourescence assay on mer-gfp construct
Flourescence Assay Protocol
- Draw sample of 1.5 ml from incubated cells with Hg
- Plot OD's
- Spin down 3 mins at 13000 rpm
- Aspirate and resuspend in 1.5 ml .9% NaCl
- Repeat 3
- Repeat 4
- Put 150ul in plate reader
