IGEM:MIT/2007/Notebook/2007-8-1: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→Agenda) |
(→Agenda) |
||
| Line 1: | Line 1: | ||
=Agenda= | =Agenda= | ||
#Decide on constitutive promoter for CPX | |||
#*Transform promoter into DH5a | |||
#Check sequencing results for Mer/GFP 3A | |||
#Perform flourescence assay on mer-gfp construct | |||
==Flourescence Assay Protocol== | |||
===Flourescence Assay Protocol=== | |||
# Draw sample of 1.5 ml from incubated cells with Hg | # Draw sample of 1.5 ml from incubated cells with Hg | ||
# Plot OD's | # Plot OD's | ||
| Line 13: | Line 14: | ||
# Repeat 4 | # Repeat 4 | ||
# Put 150ul in plate reader | # Put 150ul in plate reader | ||
===Sequencing Results=== | |||
*mer.I13500 in pSB1AT3 came back really crappy. Biobrick prefix and suffix are partially present, cannot locate the sequence for GFP. | |||
*Need to send in the parts for sequencing. | |||
*Will run a colony PCR as a diagnostic. | |||
Revision as of 09:33, 1 August 2007
Agenda
- Decide on constitutive promoter for CPX
- Transform promoter into DH5a
- Check sequencing results for Mer/GFP 3A
- Perform flourescence assay on mer-gfp construct
Flourescence Assay Protocol
- Draw sample of 1.5 ml from incubated cells with Hg
- Plot OD's
- Spin down 3 mins at 13000 rpm
- Aspirate and resuspend in 1.5 ml .9% NaCl
- Repeat 3
- Repeat 4
- Put 150ul in plate reader
Sequencing Results
- mer.I13500 in pSB1AT3 came back really crappy. Biobrick prefix and suffix are partially present, cannot locate the sequence for GFP.
- Need to send in the parts for sequencing.
- Will run a colony PCR as a diagnostic.
