IGEM:MIT/2007/Notebook/2007-8-1

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(→Flourescence Assay Protocol)
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# Repeat 4
# Repeat 4
# Put 150ul in plate reader
# Put 150ul in plate reader
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<gallery>
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Image:8-1-AHL-5Ai.jpg|AHL 5Ai
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Image:8-1-AHL-5Aii.jpg|AHL 5Aii
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Image:8-1-AHL-5Bi.jpg|AHL 5Bi
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Image:8-1-AHL-5Bii.jpg|AHL 5Bii
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Image:8-1-AHL-6Ai.jpg|AHL 6Ai
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Image:8-1-AHL-6Aii.jpg|AHL 6Aii
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Image:8-1-AHL-6Bi.jpg|AHL 6Bi
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Image:8-1-AHL-7Ai.jpg|AHL 7Ai
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Image:8-1-AHL-7Aii.jpg|AHL 7Aii
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Image:8-1-AHL-7Aiii.jpg|AHL 7Aiii
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Image:8-1-AHL-7Bi.jpg|AHL 7Bi
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Image:8-1-AHL-7Bii.jpg|AHL 7Bii
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Image:8-1-AHL-8Ai.jpg|AHL 8Ai
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Image:8-1-AHL-8Aii.jpg|AHL 8Aii
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Image:8-1-AHL-8Bi.jpg|AHL 8Bi
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Image:8-1-AHL-8Bii.jpg|AHL 8Bii
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Image:8-1-controlAi.jpg|Control Ai
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Image:8-1-controlAii.jpg|Control Aii
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Image:8-1-controlBi.jpg|Control Bi
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Image:8-1-controlBii.jpg|Control Bii
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</gallery>
===Sequencing Results===
===Sequencing Results===

Revision as of 11:07, 2 August 2007

Agenda

  1. Decide on constitutive promoter for CPX
    • Transform promoter into DH5a
  2. Check sequencing results for Mer/GFP 3A
  3. Perform flourescence assay on mer-gfp construct


Flourescence Assay Protocol

  1. Draw sample of 1.5 ml from incubated cells with Hg
  2. Plot OD's
  3. Spin down 3 mins at 13000 rpm
  4. Aspirate and resuspend in 1.5 ml .9% NaCl
  5. Repeat 3
  6. Repeat 4
  7. Put 150ul in plate reader


AHL 5Ai

AHL 5Aii

AHL 5Bi

AHL 5Bii

AHL 6Ai

AHL 6Aii

AHL 6Bi

AHL 7Ai

AHL 7Aii

AHL 7Aiii

AHL 7Bi

AHL 7Bii

AHL 8Ai

AHL 8Aii

AHL 8Bi

AHL 8Bii

Control Ai

Control Aii

Control Bi

Control Bii

Sequencing Results

  • mer.I13500 in pSB1AT3 came back really crappy. Biobrick prefix and suffix are partially present, cannot locate the sequence for GFP.
  • Need to send in the parts for sequencing.
  • Will run a colony PCR as a diagnostic.
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