IGEM:MIT/2007/Notebook/2007-8-1

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(Agenda)
(Flourescence Assay Protocol)
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# Repeat 4
# Repeat 4
# Put 150ul in plate reader
# Put 150ul in plate reader
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<gallery>
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Image:8-1-AHL-5Ai.jpg|AHL 5Ai
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Image:8-1-AHL-5Aii.jpg|AHL 5Aii
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Image:8-1-AHL-5Bi.jpg|AHL 5Bi
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Image:8-1-AHL-5Bii.jpg|AHL 5Bii
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Image:8-1-AHL-6Ai.jpg|AHL 6Ai
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Image:8-1-AHL-6Aii.jpg|AHL 6Aii
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Image:8-1-AHL-6Bi.jpg|AHL 6Bi
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Image:8-1-AHL-7Ai.jpg|AHL 7Ai
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Image:8-1-AHL-7Aii.jpg|AHL 7Aii
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Image:8-1-AHL-7Aiii.jpg|AHL 7Aiii
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Image:8-1-AHL-7Bi.jpg|AHL 7Bi
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Image:8-1-AHL-7Bii.jpg|AHL 7Bii
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Image:8-1-AHL-8Ai.jpg|AHL 8Ai
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Image:8-1-AHL-8Aii.jpg|AHL 8Aii
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Image:8-1-AHL-8Bi.jpg|AHL 8Bi
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Image:8-1-AHL-8Bii.jpg|AHL 8Bii
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Image:8-1-controlAi.jpg|Control Ai
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Image:8-1-controlAii.jpg|Control Aii
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Image:8-1-controlBi.jpg|Control Bi
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Image:8-1-controlBii.jpg|Control Bii
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</gallery>
===Sequencing Results===
===Sequencing Results===

Revision as of 11:07, 2 August 2007

Agenda

  1. Decide on constitutive promoter for CPX
    • Transform promoter into DH5a
  2. Check sequencing results for Mer/GFP 3A
  3. Perform flourescence assay on mer-gfp construct


Flourescence Assay Protocol

  1. Draw sample of 1.5 ml from incubated cells with Hg
  2. Plot OD's
  3. Spin down 3 mins at 13000 rpm
  4. Aspirate and resuspend in 1.5 ml .9% NaCl
  5. Repeat 3
  6. Repeat 4
  7. Put 150ul in plate reader


Sequencing Results

  • mer.I13500 in pSB1AT3 came back really crappy. Biobrick prefix and suffix are partially present, cannot locate the sequence for GFP.
  • Need to send in the parts for sequencing.
  • Will run a colony PCR as a diagnostic.
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