IGEM:MIT/2007/Notebook/2007-8-10: Difference between revisions
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(New page: ==Agenda== #Run Western Blot (at Belcher Lab with Rana and Robbie) ==Western Blot== Lanes: <pre> 1 Invitrogen BenchMark Ladder 2 T 1E-5 3 T 1E-7 4 T 1E-8 5 T 0 6 U 1E-5 7 U 1...) |
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Numbers = concentration of AHL added to culture to induce transcription | Numbers = concentration of AHL added to culture to induce transcription | ||
[[Image:westernblotCPX.jpg|thumb|400px|''Figure 1''': Western Blot of DH5a expressing CPX]] | |||
Revision as of 15:27, 10 August 2007
Agenda
- Run Western Blot (at Belcher Lab with Rana and Robbie)
Western Blot
Lanes:
1 Invitrogen BenchMark Ladder 2 T 1E-5 3 T 1E-7 4 T 1E-8 5 T 0 6 U 1E-5 7 U 1E-8 8 U 0 9 Positive Control
Key:
T = DH5a transformed with F2620.B0034.CPX in plasmid pSB1AC3
U = DH5a untransformed
Numbers = concentration of AHL added to culture to induce transcription
