IGEM:MIT/2007/Notebook/2007-8-10

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(New page: ==Agenda== #Run Western Blot (at Belcher Lab with Rana and Robbie) ==Western Blot== Lanes: <pre> 1 Invitrogen BenchMark Ladder 2 T 1E-5 3 T 1E-7 4 T 1E-8 5 T 0 6 U 1E-5 7 U 1...)
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Numbers = concentration of AHL added to culture to induce transcription
Numbers = concentration of AHL added to culture to induce transcription
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[[Image:westernblotCPX.jpg|thumb|400px|''Figure 1''': Western Blot of DH5a expressing CPX]]

Revision as of 17:27, 10 August 2007

Agenda

  1. Run Western Blot (at Belcher Lab with Rana and Robbie)

Western Blot

Lanes:

1  Invitrogen BenchMark Ladder
2  T  1E-5
3  T  1E-7
4  T  1E-8
5  T  0
6  U  1E-5
7  U  1E-8
8  U  0
9  Positive Control

Key:

T = DH5a transformed with F2620.B0034.CPX in plasmid pSB1AC3

U = DH5a untransformed

Numbers = concentration of AHL added to culture to induce transcription


Figure 1': Western Blot of DH5a expressing CPX
Figure 1': Western Blot of DH5a expressing CPX
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