IGEM:MIT/2007/Notebook/2007-8-10

Agenda

1. Run Western Blot (at Belcher Lab with Rana and Robbie)

Western Blot

Lanes:

1  Invitrogen BenchMark Ladder
2  T  1E-5
3  T  1E-7
4  T  1E-8
5  T  0
6  U  1E-5
7  U  1E-8
8  U  0
9  Positive Control

Key:

T = DH5a transformed with F2620.B0034.CPX in plasmid pSB1AC3

U = DH5a untransformed

Numbers = concentration of AHL added to culture to induce transcription

Figure 1': Western Blot of DH5a expressing CPX

Figure 2': Western Blot of DH5a expressing CPX

Figure 3': Western Blot of DH5a expressing CPX