IGEM:MIT/2007/Notebook/2007-8-11
From OpenWetWare
(Difference between revisions)
(→Agenda) |
(→Agenda) |
||
| Line 1: | Line 1: | ||
==Agenda== | ==Agenda== | ||
#Double digestion: | #Double digestion: | ||
| - | #*R0051.B0034, I14032.B0034, CPX.B0014 - for 3A assembly into pSB1AC3 | + | #*R0051.B0034, I14032.B0034, CPX.B0014 - for 3A assembly into pSB1AC3. |
#*F2620.B0034.CPX, B0014 - for standard assembly and to figure out extra bands in western blot | #*F2620.B0034.CPX, B0014 - for standard assembly and to figure out extra bands in western blot | ||
#Purify digestion reactions. Remember to use <b>BUFFER TE</b> and NOT H2O. | #Purify digestion reactions. Remember to use <b>BUFFER TE</b> and NOT H2O. | ||
| Line 9: | Line 9: | ||
#*F2620.B0034.CPX.B0014 | #*F2620.B0034.CPX.B0014 | ||
#Transform ligations into DH5a and plate. | #Transform ligations into DH5a and plate. | ||
| + | |||
| + | ==Holding off on constitutive promoter digestion/purification/ligation/transformation until sequencing results come back== | ||
Revision as of 12:25, 11 August 2007
Agenda
- Double digestion:
- R0051.B0034, I14032.B0034, CPX.B0014 - for 3A assembly into pSB1AC3.
- F2620.B0034.CPX, B0014 - for standard assembly and to figure out extra bands in western blot
- Purify digestion reactions. Remember to use BUFFER TE and NOT H2O.
- Ligations of digestion mixtures
- R0051.B0034.CPX.B0014
- I14032.B0034.CPX.B0014
- F2620.B0034.CPX.B0014
- Transform ligations into DH5a and plate.
