IGEM:MIT/2007/Notebook/2007-8-12: Difference between revisions
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<pre> | <pre> | ||
Plate Plate Marker Count Notes | Plate Plate Marker Count Notes | ||
insert only | insert only Cm 0 | ||
insert only | insert only Kan 3640 very small | ||
cPCR #6 Cm 240 very small | |||
cPCR #6 | cPCR #6 Kan 532 normal sized | ||
cPCR #6 (-) control vector only | cPCR #6 (-) control vector only Cm 26 very small | ||
cPCR #6 (-) control vector only | cPCR #6 (-) control vector only Kan 0 | ||
cPCR #8 Cm 1600 very small | |||
cPCR #8 | cPCR #8 Kan 2400 normal size near center, very small on peripheral | ||
cPCR #8 (-) control vector only | cPCR #8 (-) control vector only Cm 11 very small | ||
cPCR #8 (-) control vector only | cPCR #8 (-) control vector only Kan 5 very small | ||
</pre> | </pre> | ||
===LC'ed 6 colonies each from cPCR #6 & #8 S.A. ligation of F+B+CPX+B0014 into pSB1AC3 in B-R cells=== | ===LC'ed 6 colonies each from cPCR #6 & #8 S.A. ligation of F+B+CPX+B0014 into pSB1AC3 in B-R cells=== | ||
*37C @ 10pm | *37C @ 10pm | ||
Revision as of 19:25, 12 August 2007
Removed Transformation Plates from 37C - Colony Count
Plate Plate Marker Count Notes insert only Cm 0 insert only Kan 3640 very small cPCR #6 Cm 240 very small cPCR #6 Kan 532 normal sized cPCR #6 (-) control vector only Cm 26 very small cPCR #6 (-) control vector only Kan 0 cPCR #8 Cm 1600 very small cPCR #8 Kan 2400 normal size near center, very small on peripheral cPCR #8 (-) control vector only Cm 11 very small cPCR #8 (-) control vector only Kan 5 very small
LC'ed 6 colonies each from cPCR #6 & #8 S.A. ligation of F+B+CPX+B0014 into pSB1AC3 in B-R cells
- 37C @ 10pm
