IGEM:MIT/2007/Notebook/2007-8-12

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Removed Transformation Plates from 37C - Colony Count

Plate                               Plate Marker        Count        Notes
insert only                              Cm               0  
insert only                              Kan              3640       very small
**cPCR #6                                Cm               240        very small
cPCR #6                                  Kan              532        normal sized
cPCR #6 (-) control vector only          Cm               26         very small
cPCR #6 (-) control vector only          Kan              0
**cPCR #8                                Cm               1600       very small
cPCR #8                                  Kan              2400       normal size near center, very small on peripheral
cPCR #8 (-) control vector only          Cm               11         very small
cPCR #8 (-) control vector only          Kan              5          very small

LC'ed 6 colonies each from cPCR #6 & #8 S.A. ligation of F+B+CPX+B0014 into pSB1AC3 in B-R cells

  • 37C @ 10pm
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