IGEM:MIT/2007/Notebook/2007-8-15

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(New page: ==Mercury Removal Assay Protocol== * Derived from Bae et al. Papers # Grow to OD of .3 in LB + Amp # Induce with 1mM IPTG # Induce for 2,3,4,5 hrs # Grow to OD of 1.0 # Suspend in LB mediu...)
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Revision as of 11:10, 14 August 2007

Mercury Removal Assay Protocol

  • Derived from Bae et al. Papers
  1. Grow to OD of .3 in LB + Amp
  2. Induce with 1mM IPTG
  3. Induce for 2,3,4,5 hrs
  4. Grow to OD of 1.0
  5. Suspend in LB medium w/ 5uM Hg2+
  6. Check [Hg2+] after 1 hr
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