IGEM:MIT/2007/Notebook/2007-8-15: Difference between revisions
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(New page: ==Mercury Removal Assay Protocol== * Derived from Bae et al. Papers # Grow to OD of .3 in LB + Amp # Induce with 1mM IPTG # Induce for 2,3,4,5 hrs # Grow to OD of 1.0 # Suspend in LB mediu...) |
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==Agenda== | |||
#Make more DD R0051 | |||
#Redo R0051.B0034 ligation | |||
#Get electrocompetent cells from TK | |||
#Transform R0051.B0034 and plate | |||
#Transform I14032.B0034.CPX.B0014 into competent cells and plate | |||
#Run polystyrene-binding efficiency assay | |||
#Mercury removal assay | |||
==Mercury Removal Assay Protocol== | ==Mercury Removal Assay Protocol== | ||
* Derived from Bae et al. Papers | * Derived from Bae et al. Papers | ||
Revision as of 14:33, 14 August 2007
Agenda
- Make more DD R0051
- Redo R0051.B0034 ligation
- Get electrocompetent cells from TK
- Transform R0051.B0034 and plate
- Transform I14032.B0034.CPX.B0014 into competent cells and plate
- Run polystyrene-binding efficiency assay
- Mercury removal assay
Mercury Removal Assay Protocol
- Derived from Bae et al. Papers
- Grow to OD of .3 in LB + Amp
- Induce with 1mM IPTG
- Induce for 2,3,4,5 hrs
- Grow to OD of 1.0
- Suspend in LB medium w/ 5uM Hg2+
- Check [Hg2+] after 1 hr
