IGEM:MIT/2007/Notebook/2007-8-15: Difference between revisions
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==Agenda== | ==Agenda== | ||
#Make electrocompetent BL-21 cells | |||
#Make more DD R0051 | #Make more DD R0051 | ||
#Redo R0051.B0034 ligation | #Redo R0051.B0034 ligation |
Revision as of 07:13, 15 August 2007
Agenda
- Make electrocompetent BL-21 cells
- Make more DD R0051
- Redo R0051.B0034 ligation
- Get electrocompetent cells from TK
- Transform R0051.B0034 and plate
- Transform I14032.B0034.CPX.B0014 into competent cells and plate
- Run polystyrene-binding efficiency assay
- Mercury removal assay
Mercury Removal Assay Protocol
- Derived from Bae et al. Papers
- Grow to OD of .3 in LB + Amp
- Induce with 1mM IPTG
- Induce for 2,3,4,5 hrs
- Grow to OD of 1.0
- Suspend in LB medium w/ 5uM Hg2+
- Check [Hg2+] after 1 hr