IGEM:MIT/2007/Notebook/2007-8-16

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(Agenda)
Line 7: Line 7:
#DD I14032.B0034 and put it into pSB1AC3 as a blank vector
#DD I14032.B0034 and put it into pSB1AC3 as a blank vector
#Run Polystyrene binding efficiency assay
#Run Polystyrene binding efficiency assay
 +
 +
==Making Electrochemically competent cells==
 +
Using BL-21 (for higher protein expression) Followed TK's OWW protocol.
 +
 +
==DD I14032.B0034 Mixture==
 +
5 µL    I14032.B0034 #4 (225.7 ng/µL)
 +
1 µL    EcoRI
 +
1 µL    PstI
 +
0.5 µL  BSA
 +
5 µL    NEB Buffer 3
 +
37.5 µL  H20
 +
 +
put into 37C at 12:15 PM

Revision as of 12:24, 16 August 2007

Agenda

  1. Make electro/chemically competent cells
  2. Transform and plate R0051
  3. Make more DD R0051
  4. Redo R0051.B0034 ligation
  5. DD I14032.B0034 and put it into pSB1AC3 as a blank vector
  6. Run Polystyrene binding efficiency assay

Making Electrochemically competent cells

Using BL-21 (for higher protein expression) Followed TK's OWW protocol.

DD I14032.B0034 Mixture

5 µL I14032.B0034 #4 (225.7 ng/µL) 1 µL EcoRI 1 µL PstI 0.5 µL BSA 5 µL NEB Buffer 3 37.5 µL H20

put into 37C at 12:15 PM

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