IGEM:MIT/2007/Notebook/2007-8-22

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Agenda)
Line 9: Line 9:
*Transform reporter gene for mercury sensing system (Biobrick) - need to decide on which one first!
*Transform reporter gene for mercury sensing system (Biobrick) - need to decide on which one first!
**eventually must ligate it with an RBS?
**eventually must ligate it with an RBS?
 +
 +
==Wrapped Up Making iGEM Antibiotic Stocks==
 +
*Apparently iGEM needed to make own antibiotic stocks, so we did (SK)
 +
*All are stored in -20 iGEM freezer, in two boxes
 +
**Eppendorf box has A, Cm, K, T in 1 mL aliquots
 +
**Blue box has large aliquots of A, Cm, K, T in Falcon tubes and remaining 1 mL aliquots
 +
*Protocol from Francois: http://openwetware.org/wiki/Endy:Preparing_Antibiotic_Stocks
 +
**However, Cm protocol was made by SK -- not yet on OWW

Revision as of 17:41, 22 August 2007

Agenda

  • Clean out fridge: Threw out contaminated/overgrown plates, added fresh LB to LC's
  • Made glycerol stock of F2620+B0034+CPX+B0014 (for B-r cells cPCR6-1 and cPCR8-5, for BL21 cPCR6-1a, cPCR6-1b)
  • LC'ed yesterday's plating of transformations

Uncompleted:

  • Design Mer Primer
  • Transform PTO40
  • Transform reporter gene for mercury sensing system (Biobrick) - need to decide on which one first!
    • eventually must ligate it with an RBS?

Wrapped Up Making iGEM Antibiotic Stocks

  • Apparently iGEM needed to make own antibiotic stocks, so we did (SK)
  • All are stored in -20 iGEM freezer, in two boxes
    • Eppendorf box has A, Cm, K, T in 1 mL aliquots
    • Blue box has large aliquots of A, Cm, K, T in Falcon tubes and remaining 1 mL aliquots
  • Protocol from Francois: http://openwetware.org/wiki/Endy:Preparing_Antibiotic_Stocks
    • However, Cm protocol was made by SK -- not yet on OWW
Personal tools