IGEM:MIT/2007/Notebook/2007-8-22

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Agenda

  • Clean out fridge: Threw out contaminated/overgrown plates, added fresh LB to LC's
  • Made glycerol stock of F2620+B0034+CPX+B0014 (for B-r cells cPCR6-1 and cPCR8-5, for BL21 cPCR6-1a, cPCR6-1b)
  • LC'ed yesterday's plating of transformations

Uncompleted:

  • Design Mer Primer
  • Transform PTO40
  • Transform reporter gene for mercury sensing system (Biobrick) - need to decide on which one first!
    • eventually must ligate it with an RBS?