IGEM:MIT/2007/Notebook/2007-8-22

Agenda

• Clean out fridge: Threw out contaminated/overgrown plates, added fresh LB to LC's
• Made glycerol stock of F2620+B0034+CPX+B0014 (for B-r cells cPCR6-1 and cPCR8-5, for BL21 cPCR6-1a, cPCR6-1b)
• LC'ed yesterday's plating of transformations

Uncompleted:

• Design Mer Primer
• Transform PTO40
• Transform reporter gene for mercury sensing system (Biobrick) - need to decide on which one first!
  • eventually must ligate it with an RBS?

Wrapped Up Making iGEM Antibiotic Stocks

• Apparently iGEM needed to make own antibiotic stocks, so we did (SK)
• All are stored in -20 iGEM freezer, in two boxes
  • Eppendorf box has A, Cm, K, T in 1 mL aliquots
  • Blue box has large aliquots of A, Cm, K, T in Falcon tubes and remaining 1 mL aliquots
• Protocol from Francois: http://openwetware.org/wiki/Endy:Preparing_Antibiotic_Stocks
  • However, Cm protocol was made by SK -- not yet on OWW