IGEM:MIT/2007/Notebook/2007-8-3

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(New page: ===Took Out LC's of I14032, B0014, R0051=== *1ml for glycerol stock *rest miniprepped (for sequencing and 3A ligation) **when spun down, B0014 cells slightly reddish tint?)
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*rest miniprepped (for sequencing and 3A ligation)
*rest miniprepped (for sequencing and 3A ligation)
**when spun down, B0014 cells slightly reddish tint?
**when spun down, B0014 cells slightly reddish tint?
 +
 +
 +
==Transformations==
 +
*Transformed DH5 alpha with the mer operon and the gfp part to send for sequencing
 +
*PCR mer operon in psb3k3
 +
*I3500 in psb3k3

Revision as of 11:01, 3 August 2007

Took Out LC's of I14032, B0014, R0051

  • 1ml for glycerol stock
  • rest miniprepped (for sequencing and 3A ligation)
    • when spun down, B0014 cells slightly reddish tint?


Transformations

  • Transformed DH5 alpha with the mer operon and the gfp part to send for sequencing
  • PCR mer operon in psb3k3
  • I3500 in psb3k3
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