IGEM:MIT/2007/Notebook/2007-8-4: Difference between revisions
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Revision as of 06:53, 7 August 2007
Double Digests!!!
5.3 µl I14032 ( 188.4 ng/µl) 0.5 µl BSA 5 µl NEB2 Buffer 1 µl SpeI 1 µl PstI 37.7 µl H20 -------------------------- 50 µl Total 42.5 µl R0051 (16.8 ng/µl) 0.5 µl BSA 5 µl NEB2 Buffer 1 µl SpeI 1 µl PstI -------------------------- 50 µl Total 12 µl B0034 (84.9 ng/µl) 0.5 µl BSA 5 µl NEB3 Buffer 1 µl XbaI 1 µl PstI 30.5 µl H2O -------------------------- 50 µl Total 5 µl CPX (1µg/5µl) 0.5 µl BSA 5 µl EcoRI Buffer 1 µl EcoRI 1 µl SpeI 37.5 µl H2O -------------------------- 50 µl Total 16 µl B0014 (62.8 ng/µl) 0.5 µl BSA 5 µl NEB2 Buffer 1 µl XbaI 1 µl EcoRI 26.5 µl H2O -------------------------- 50 µl Total
